Handbook of Culture Media for Food and Water Microbiology by janet e. l. (editor) ; curtis, gordon d. w. (editor) ; PDF

By janet e. l. (editor) ; curtis, gordon d. w. (editor) ; baird, rosamund m. (editor) corry

ISBN-10: 1847559166

ISBN-13: 9781847559166

This is the hugely expected 3rd variation of a publication written through the operating get together on tradition Media of the foreign Committee on nutrition Microbiology and Hygiene. it's a convenient reference for microbiologists eager to be aware of which media to take advantage of for the detection of assorted teams of microbes in meals and the way to examine the functionality of the media. The e-book is split into elements and concentrates on media for water in addition to nutrients microbes - determining these which were evaluated and proven to operate optimally. the 1st half includes a sequence of chapters written through a number of specialists from worldwide, reviewing the media designed to realize the foremost teams of microbes very important in meals spoilage, nutrients fermentations and food-borne ailment. The background and intent of the selective brokers and indicator platforms used, in addition to the relative benefits of many of the media are surveyed by way of connection with the medical literature. the second one half comprises monographs on virtually a hundred of the media thought of most precious. every one monograph, written within the type of a pharmacopoeia, comprises: a quick part at the heritage and selective precept of the medium; a style for its practise from easy materials; its visual appeal and actual houses, together with pH; its shelf-life; directions referring to approach to inoculation, incubation and interpretation; the steered method(s) and an inventory of try out lines appropriate for assessing the standard (productivity and selectivity) of the medium and an outline of the common visual appeal of the objective organism.

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janet e. l. (editor) ; curtis, gordon d. w. (editor) ;'s Handbook of Culture Media for Food and Water Microbiology PDF

This can be the hugely expected 3rd variation of a e-book written by way of the operating celebration on tradition Media of the foreign Committee on nutrition Microbiology and Hygiene. it's a convenient reference for microbiologists desirous to recognize which media to take advantage of for the detection of varied teams of microbes in meals and the way to envision the functionality of the media.

Extra info for Handbook of Culture Media for Food and Water Microbiology

Sample text

A very useful alternative approach is to test for equivalence, rather than difference, in performance, as described by Altman and Bland (1987), but this requires several pairs of results and cannot be used on individual results. 4 Selectivity Factor and Specificity Selectivity can be defined as the ‘‘difference in cfu counts determined by inoculation of a selective culture medium and a non-selective reference medium with an appropriate inoculum of a defined test microorganism’’. Selectivity has to achieve defined values for a particular organism or group of organisms.

Such precision limits can be used to determine the combination of numbers of plates and the numbers of colonies to be counted in order to optimise the precision of the test. Although the theoretical distribution of colonies conforms to Poisson, in practice this may not be the case due to cell aggregation (clumping) and the presence of non-viable cells. It is therefore desirable to test the numbers of cfu counted for compliance with a Poisson distribution and, if necessary, to ‘‘normalise’’ the number of cfu recorded for each set of plates, by a suitable mathematical transformation.

1987; ISO, 2008). , 1998; ISO, 2003c, 2008) define the ability of a culture medium to inhibit undesirable strains or to select for desirable strains, respectively. , 1991). , 1973). , 1991). The pour plate technique is not favoured, because it does not allow proper analysis of the elective properties of a solid medium, although an exception may be necessary for the assessment of media for fastidious or facultative anaerobes. For instance, Payne et al. (1999) have reported that with some, but not all, culture media tested, the pour plate technique yielded higher numbers of bifidobacteria colonies than were obtained using spread plate techniques.

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Handbook of Culture Media for Food and Water Microbiology by janet e. l. (editor) ; curtis, gordon d. w. (editor) ; baird, rosamund m. (editor) corry


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