By janet e. l. (editor) ; curtis, gordon d. w. (editor) ; baird, rosamund m. (editor) corry
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Extra info for Handbook of Culture Media for Food and Water Microbiology
A very useful alternative approach is to test for equivalence, rather than difference, in performance, as described by Altman and Bland (1987), but this requires several pairs of results and cannot be used on individual results. 4 Selectivity Factor and Speciﬁcity Selectivity can be deﬁned as the ‘‘diﬀerence in cfu counts determined by inoculation of a selective culture medium and a non-selective reference medium with an appropriate inoculum of a deﬁned test microorganism’’. Selectivity has to achieve deﬁned values for a particular organism or group of organisms.
Such precision limits can be used to determine the combination of numbers of plates and the numbers of colonies to be counted in order to optimise the precision of the test. Although the theoretical distribution of colonies conforms to Poisson, in practice this may not be the case due to cell aggregation (clumping) and the presence of non-viable cells. It is therefore desirable to test the numbers of cfu counted for compliance with a Poisson distribution and, if necessary, to ‘‘normalise’’ the number of cfu recorded for each set of plates, by a suitable mathematical transformation.
1987; ISO, 2008). , 1998; ISO, 2003c, 2008) deﬁne the ability of a culture medium to inhibit undesirable strains or to select for desirable strains, respectively. , 1991). , 1973). , 1991). The pour plate technique is not favoured, because it does not allow proper analysis of the elective properties of a solid medium, although an exception may be necessary for the assessment of media for fastidious or facultative anaerobes. For instance, Payne et al. (1999) have reported that with some, but not all, culture media tested, the pour plate technique yielded higher numbers of biﬁdobacteria colonies than were obtained using spread plate techniques.
Handbook of Culture Media for Food and Water Microbiology by janet e. l. (editor) ; curtis, gordon d. w. (editor) ; baird, rosamund m. (editor) corry