By Heather Bowe, Kylie Martin, Howard Manns
Conversation throughout Cultures continues to be a good source for college kids of linguistics and similar disciplines, together with anthropology, sociology and schooling. it's also a helpful source for pros curious about language and intercultural communique during this worldwide period.
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Additional info for Communication Across Cultures: Mutual Understanding in a Global World (2nd Edition)
Terminate the collagenase digestion by discarding the medium (check that there is no evidence of cell detachment at this stage). Gently rinse the cell layer twice with 10 mL of Ca2+- and Mg2+-free PBS. 4. , step 3. 5–4 × 106 cells/75-cm2 flask after 28 d in primary culture. Cell viability is generally ≥90%. 2. Osteoblastic Cell Lines Osteosarcomas are malignant tumors of bone derived from cells of the osteoblast lineage. An osteosarcoma is a relatively uncommon tumor, although it is the most frequently occurring primary malignant tumor of bone, predominantly occurring in children and adolescents.
J. Invest. Dermatol. Symp. Proc. 1, 188–194. 43. Valyi-Nagy, I. and Herlyn, M. ), Kluwer Academic, Boston, MA, pp. 85–101. 44. 44 Scott, G. A. and Haake, A. R. (1991) Keratinocytes regulate melanocyte number in human fetal and neonatal skin equivalents. J. Invest. Dermatol. 97, 776–781. Normal Human Epidermal Melanocytes 27 45. , Franzi, A. , and Cancedda, R. (1988) Human epithelial cells induce human melanocyte growth in vitro but only skin keratinocytes regulate its proper differentiation in the absence of dermis.
Remove the flasks from the incubator and examine under the microscope. Look for the presence of rounded, highly refractile cell bodies floating in the trypsinEDTA solution. If none, or only a few, are visible, tap the base of the flask sharply on the bench top in an effort to dislodge the cells. If this is without effect, incubate the cells for a further 5 min at 37°C. 5. When the bulk of the cells have become detached from the culture substratum, transfer the cells to a “Universal container” with 10 mL of DMEM with 10% FCS to inhibit tryptic activity.
Communication Across Cultures: Mutual Understanding in a Global World (2nd Edition) by Heather Bowe, Kylie Martin, Howard Manns