Cell Culture and its Application - download pdf or read online

By Ronald T. Acton

ISBN-10: 0120430509

ISBN-13: 9780120430505

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It does not 2 have any significant sulfation factor activity , and it does not compete for the receptor site for Epidermal Growth Factor^. No esterase activity was detected, even at FGF concentrations as high as 1 mg/ml with either denatured bovine serum albumin or Ν α -benzoyl-L-arginine ethyl ester as substrates. 005%. Treatment with proteolytic enzymes such as trypsin, chymotrypsin, pepsin, or proteases destroyed 90% of the FGF activity Β. , 1976a). Effect of Brain and Pituitary FGF on the Proliferation of 3T3 Cells The addition of brain or pituitary FGF to resting populations of Balb/c 3T3 cells results in the initiation of DNA synthesis (Gospodarowicz, 1974).

Exp. Med. 141, 1376-1389. Goldstein, G. (1968). Lancet 2, 119-122. Goldstein, G. (1974). Nature 247, 11^14. Goldstein, G. (1975). Ann. Y. Acad. Sei. 249, 177-183. Goldstein, A. , Zatz, Μ. , Hardy, M. A. and White, DIFFERENTIATION OF Τ CELLS 47 Α. (19721. proc, Nat, Acad. A, 69, 1800. , Boyse, Ε. , Schlesin^ ger, D . H. and Niall, H. D . Ü-975]. Proc. Nat. Acad. Sei. S. Α. 72, 11-15. Goldstein, G. and Schlesinger, D. H. (1975). Lancet 2, 256-259. , Boyse, E. A. and Old, L. J. (1973). J. Exp. Med.

Thus we infer that ubiquitin engages widespread ß-adrenergic receptors linked to adenylate cyclase and thus triggers induction of differentiation in a number of precommitted precursor cells, each type manifesting its precommitted program. Thymopoietin re- ceptors, which would be discreet from the 3-adrenergic receptors engaged by ubiquitin, would likely be restricted to the prothymocyte, and thus thymopoietin would selectively induce elevations of intracellular cyclic AMP in this class of precommitted precursor cells.

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Cell Culture and its Application by Ronald T. Acton


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