By Ronald T. Acton
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Extra resources for Cell Culture and its Application
It does not 2 have any significant sulfation factor activity , and it does not compete for the receptor site for Epidermal Growth Factor^. No esterase activity was detected, even at FGF concentrations as high as 1 mg/ml with either denatured bovine serum albumin or Ν α -benzoyl-L-arginine ethyl ester as substrates. 005%. Treatment with proteolytic enzymes such as trypsin, chymotrypsin, pepsin, or proteases destroyed 90% of the FGF activity Β. , 1976a). Effect of Brain and Pituitary FGF on the Proliferation of 3T3 Cells The addition of brain or pituitary FGF to resting populations of Balb/c 3T3 cells results in the initiation of DNA synthesis (Gospodarowicz, 1974).
Exp. Med. 141, 1376-1389. Goldstein, G. (1968). Lancet 2, 119-122. Goldstein, G. (1974). Nature 247, 11^14. Goldstein, G. (1975). Ann. Y. Acad. Sei. 249, 177-183. Goldstein, A. , Zatz, Μ. , Hardy, M. A. and White, DIFFERENTIATION OF Τ CELLS 47 Α. (19721. proc, Nat, Acad. A, 69, 1800. , Boyse, Ε. , Schlesin^ ger, D . H. and Niall, H. D . Ü-975]. Proc. Nat. Acad. Sei. S. Α. 72, 11-15. Goldstein, G. and Schlesinger, D. H. (1975). Lancet 2, 256-259. , Boyse, E. A. and Old, L. J. (1973). J. Exp. Med.
Thus we infer that ubiquitin engages widespread ß-adrenergic receptors linked to adenylate cyclase and thus triggers induction of differentiation in a number of precommitted precursor cells, each type manifesting its precommitted program. Thymopoietin re- ceptors, which would be discreet from the 3-adrenergic receptors engaged by ubiquitin, would likely be restricted to the prothymocyte, and thus thymopoietin would selectively induce elevations of intracellular cyclic AMP in this class of precommitted precursor cells.
Cell Culture and its Application by Ronald T. Acton