Download e-book for kindle: Biophysical Tools for Biologists, Volume Two: In Vivo by Dr. John J. Correia and Dr. H. William Detrich, III (Eds.)

By Dr. John J. Correia and Dr. H. William Detrich, III (Eds.)

ISBN-10: 0123725216

ISBN-13: 9780123725219

Pushed partially via the improvement of genomics, proteomics, and bioinformatics as new disciplines, there was an important resurgence of curiosity in actual the way to examine macromolecular constitution and serve as within the context of dwelling cells. This quantity in tools in mobile Biology is dedicated to biophysical ideas in vivo and their functions to mobile biology. the quantity covers methods-oriented chapters on basic in addition to state-of-the-art options in molecular and mobile biophysics. This publication is directed towards the vast viewers of telephone biologists, biophysicists, pharmacologists, and molecular biologists who hire classical and glossy biophysical applied sciences or desire to extend their services to incorporate such methods. it is going to additionally curiosity the biomedical and biotechnology groups for biophysical characterization of drug formulations sooner than FDA approval. * Describes innovations within the context of vital organic difficulties * Delineates severe steps and power pitfalls for every technique

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Additional resources for Biophysical Tools for Biologists, Volume Two: In Vivo Techniques

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DiVusion of low density lipoprotein-receptor complex on human fibroblasts. J. Cell Biol. 95, 846–852. , Hammond, A. , Hess, S. , Holowka, D. , Baird, B. , and Webb, W. W. (2007). Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles. Proc. Natl. Acad. Sci. USA 104, 3165–3170. , Hess, S. , and Webb, W. W. (2003). Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension. Nature 425, 821–824. , Hermens, W. , and Hof, M. (2003).

Biol. 298, 677–689. Wallace, M. , Ying, L. , and Klenerman, D. (2000). FRET fluctuation spectroscopy: Exploring the conformational dynamics of a DNA hairpin loop. J. Phys. Chem. B 104, 11551–11555. , and Lenne, P. F. (2005). Fluorescence correlation spectroscopy diVusion laws to probe the submicron cell membrane organization. Biophys. J. 89, 4029–4042. Webb, W. W. (1976). Applications of fluorescence correlation spectroscopy. Q. Rev. Biophys. 9, 49–68. Webb, W. W. (1981). Molecular mobility on the cell surface.

J. 84, 1977–1984. , Callaway, D. , and Maiti, S. (2003b). The amyloid beta peptide (Abeta(1–40)) is thermodynamically soluble at physiological concentrations. Biochemistry 42, 10506–10513. Shaner, N. , Campbell, R. , Steinbach, P. , Giepmans, B. N. , Palmer, A. , and Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567–1572. 34 Huimin Chen et al. Singer, S. , and Nicolson, G. L. (1972). The fluid mosaic model of the structure of cell membranes.

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Biophysical Tools for Biologists, Volume Two: In Vivo Techniques by Dr. John J. Correia and Dr. H. William Detrich, III (Eds.)

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