Download PDF by Jürgen Angerer: Biological Monitoring: Heutige und künftige Möglichkeiten in

By Jürgen Angerer

ISBN-10: 3527274103

ISBN-13: 9783527274109

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2 BPDE-DNA adducts per 108 nt). In the lung cancer studies, PCR/RFLP based analysis of CYP1A1 gene polymorphisms and GSTM1 gene deletion was carried out as described. (Cascorbi et al. 1996, Brockmöller et al. 1993, Volkenandt et al. 1993, Arand et al. 1996). An allele carrying only a T to C transition 1194 bp downstream of exon 7 in the 3l-flanking region, leading to a Mspl-restriction site (m1), was termed *2A. An allele with m1 plus a mutation in exon 7 leading to an lle/Val-exchange at codon 462 (m2) due to an A to G transition at nt 4889 was termed *2B.

The 32P postlabeling assay is the method most widely used for the analysis of DNA adducts (Talaska et al. 1992, Beach and Gupta 1992, Izzotti 1998, Phillips and Castagnaro 1999). This assay uses g-32P-labeled adenosine triphosphate to incorporate a highly radioactive reporter group into nucleotides After enzymatic hydrolysis of DNA, and the postlabeling procedure, the nucleotides are separated from normal nucleotides by TLC or HPLC, and visualized by radioautography or in-line scintillation counting, respectively.

Validation of a new fluorometric assay for benzo(a)pyrene diol-epoxide-DNA adducts in human white blood cells: comparison with 32P-postlabeling and ELISA, Carcinogenesis, 15, 557–560 (1994). , Lystad, S. , 54, 1551–1555 (1994). : Gluthatione S-transferase Mu (GST Mu) deficiency and DNA adducts in lymphocytes of smokers, Toxicology, 126, 155–162 (1998). , Santella, R. , Blackwood, A. M. : A molecular epidemiological casecontrol study of lung cancer, Cancer Epidemiol. , 4, 341–346 (1995). Van Schooten, F.

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Biological Monitoring: Heutige und künftige Möglichkeiten in der Arbeits- und Umweltmedizin (Rundgesprache Und Kolloquien) (German Edition) by Jürgen Angerer


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