By Gad Nathan
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Extra resources for Approximate tests of independence in contingency tables from complex stratified cluster samples (National Center for Health Statistics. Vital and health ... evaluation and methods research, series 2)
The effects shown in Fig. 6 are a good example of the reduction and leveling of the EOF over a wide pH range using a coated capillary (Towns and Regnier, 1992a). The pH independence of EOF was also observed with a negatively charged coating (sulfonic acid groups) (Kohr and Engelhardt, 1991). As with the coating described above, the flow is reduced to approximately that of an uncoated capillary at pH 3. This low flow rate may result in strongly cationic species not passing by the detector due to their movement in the opposite direction of EOF.
1987) showed a direct relationship between reduction in EOF and increasing molecular weight of adsorbed polyethylene glycol (PEG). Presumably, the larger molecular weight PEG shifts the plane of shear farther from the surface. The problem with adsorbed PEG coatings is that they are not equally effective in controlling the adsorption of cationic proteins. Adsorption of surfactants to octylsilane-derivatized capillaries is a more effective approach. EOF could be varied by using surfactants that differ in head group size and alkyl chain length (Towns and Regnier, 1991) much as in the case of PEG.
Manipulation of Electroosmotic Flow Electroosmotic flow can have both a positive and a negative influence upon the CE separations. Some positive aspects of EOF are i) a plug flow profile that minimizes band spreading, ii) transport of both anionic and cationic species past a single detector, and iii) transport of mobile phases through packed capillary chromatography columns. Negative aspects of EOF are i) substances often elute from electrophoresis columns before they can be fully resolved, ii) mixing is initiated in columns that have a non-uniform charge distribution, and iii) flow rate and mixing are very sensitive to surface fouling, as discussed earlier.
Approximate tests of independence in contingency tables from complex stratified cluster samples (National Center for Health Statistics. Vital and health ... evaluation and methods research, series 2) by Gad Nathan